Customised Services in Preclinical Inflammation

Anti-glomerular basement membrane (GBM) model in mice

Anti-GBM glomerulonephritis is a well-established antibody-induced glomerulonephritis model in which mice are immunized with autologous or heterologous anti-GBM antibodies to mimic human nephritis via the initiation of an autoantibody-mediated inflammatory response. This model shares a number of pathogenic mechanisms with human glomerulonephritis, including immune complex deposition, complement activation and immune cell infiltration. To investigate the mechanisms of autoimmunity and inflammation in glomerulonephritis and to test approaches to specific immune intervention, we can induce anti-GMB disease in 2 mouse strains (C57Bl.6 and DBA.1 mice) using Sheep Anti-Rat Glomeruli (GBM) Serum (PTX-001) from Probetex or Rabbit anti-Mouse GBM from Lampire Bio labs to achieve a heterologous or autologous anti-GBM nephritis model.

Fluorescence immunohistochemistry for autoantibodies deposition in kidneys` glomeruli: (A) C3 antibody; (B) C3d antibody and (C)
C3b/iC3b/C3c antibody. Scale bar 50μm.

Fluorescence immunohistochemistry for autoantibodies deposition in kidneys` glomeruli: (A) C3 antibody; (B) C3d antibody and (C) C3b/iC3b/C3c antibody. Scale bar 50μm.

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Model of GBM
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