MBP-induced EAE is induced in Lewis rats with MBP protein emulsified in Complete Freunds Adjuvant (CFA). MBP-induced EAE is a severe encephalomyelitis with an acute monophasic disease course. Disease has an onset around day 8-10 after immunization and experiment can normally be terminated around day 20. In this model, disease is not accompanied by demyelination of the CNS and there is mainly infiltration of T cells in the CNS. The model is thus suitable for studies to assess basic immunological mechanisms in regard to T cell function in autoimmune disease.